help method for protein estimation

CRH inconnue
Tue Mar 8 21:39:31 EST 1994

Hi friends,
The affi gel from Biorad according to the manufacturers instructions  the
time and efficiency of coupling of a ligand to affi gelis unstable in
aqueoous solutions.The alternmate way is to dissolve the protein ( ligand)
in an organic solvent in order to increase the efficiency of coupling . My
protein dissolves in formamide.
Is it possible to estimate the amount of protein in formamide by any other
method than measuring O.D at 280nm. And also can somebody tell how i can
get the back the protein from organic phase to the aqueous solution ?
i would appreciate if you could mail at the following address.
Thank you in advance.
Email add: 4104vsav at

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