calcium-phosphate transient transfections

Tracy Aquilla aquilla at
Wed Mar 9 14:59:03 EST 1994

We have had a problem with our usually very successful transient
transfections, they no longer work, we are back to square -1 trying to get
them to work. The PH has been checked and rechecked, now we are trying 3
different PH's, the calcium phosphate is new, the Dna is clean what other
nuances am I missing in my quest?

any help is appreciated.

lieber at

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