erase a base

[mhollowa at epo.som.sunysb.edu]

In article <hotan-080394162111 at pc10.hrfs.uiuc.edu> hotan@@uxa.cso.uiuc.edu (Douglas T.) writes:
> Does anyone have any comments on the erase a base system? 

Yes.
ARRRRRGGGGGGHHHHHH!!!
I've only tried it with the phosphorothiolate end protection and the 
results were frustrating.  Out of, perhaps, 50 transformants one or two 
were of plasmids whose ends had been protected from digestion.  The rest, 
far as I could tell, were bi-directional digestions.  I expect that if I 
had used a 3' overhang it would have worked much better.