In Vivo Transfection

Basavaraju Shankarappa bsh at MED.PITT.EDU
Wed Mar 9 09:34:46 EST 1994


You should look at recent experiments at immunization using naked
DNA.  I mean you can inject the DNA intramuscularly that contains
the gene of your interest under the control of a expressible 
promoter (doesn't have to be retroviral) and get sufficient expression
in situ.  You may want to take a look at the last years AAAS annual
meeting proceedings if you have them available.  If you need any 
more specific info, let me know.
Raj Shankarappa
bsh at med.pitt.edu

> 
> Does anyone know a way to transfect in vivo, I'm working on chick
> embryos and was wondering if I could transfect in some DNA to a select
> area.  I have retroviral vectors, but that's not going to do what I am
> trying to accomplish. 
> 
> Sue Jeffrey
> sjeffrey at mskcc.org
> 
> What do I know, I'm only a tech!
> 




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