DNA-protein interactions

Miss L.M. Fernandez lfernand at crc.ac.uk
Thu Mar 10 11:19:27 EST 1994


has anyone in the newsgroup used UV crosslinking
to characterise the size of DNA binding proteins
of interest ? a couple of the methods I've read
uses micrococcal nuclease together with DNase I
to digest unbound nucleotides after crosslinking...
other groups don't use m.nuclease ... do i need this
.. if yes, where can I get it as I cannot find it in any of the
catalogues I have

thanks in advance.

Levi Fernandez

e-mail address : L.M.Fernandez at qmw.ac.uk







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