disrupting e. coli cells

Bipin K Dalmia bipin at iastate.edu
Thu Mar 10 17:30:50 EST 1994

i've never had much success with sonication but since that is the only
piece of equipment we have at present, do you have any suggestions for
optimizing e. coli disruption with a sonicator? i've heard that some
people use lysozyme treatment before sonicating, do you have any
experience with this?? any other treatments??

bipin k. dalmia               the other night i was lying on my bed, looking
bipin at iastate.edu             up at the beautiful stars, and i said to myself, 
n2.bkd at isumvs.iastate.edu     'where the F*CK is my ROOF !!'

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