Removal of RNA from minipreps

ALAN J. SIMMONS simmoaj at
Thu Mar 10 02:19:14 EST 1994

Are there any reliable methods of removing RNA from alkaline minipreps
of plasmid DNA?  We do not have an ultracentrifuge at our institution.
The plasmid well serve as template in Promega's coupled TnT system
using SP6, so I would like to avoid RNase. I've not had very good luck
with NaOH hydrolysis of the RNA.  Any advice would be appreciated.
Alan J. Simmons			E-Mail: SIMMOAJ at WKUVX1.WKU.EDU
Biology Dept.
Western Kentucky University
Bowling Green KY 42101

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