Advantages of 33-P
nash at nrcbsa.bio.nrc.ca
Fri Mar 11 09:03:50 EST 1994
In article <1994Mar11.132809.93332 at vaxc.cc.monash.edu.au>,
<jost at vaxc.cc.monash.edu.au> wrote:
>Could anyone out there enlighten me on the advantages of 33-P over 32-P.
>I understand that 33-P is a weaker emitter than 32-P, but apart from that
>are there any other advantages, longer half-life for example.
The half-life is 25.4 days.
>Specifically, we want the use it for primer extension, so if there's anyone
>who's actually used 33-P for this application, I be happy to hear from you.
I use 33P for my primer extensions. It's a simple replacement for
32P, no hassles, nice bands, etc. I used it with BRL's Superscript II
RT to measure a bacterial transcript.
>Incidently, has anyone seen/done any methods for primer extension using a
>non-radioactively labelled primer. There are plenty of non-radioactive
>sequencing methods out there. It would just be a matter of labelling the
>primer with something that wouldn't affect mobility (sure!).
I know it can be done, but if it has to be done next to a sequencing
ladder, I couldn't be bothered blotting a sequencing gel, let alone
the cost of the membrane.
How's the weather back home?
John Nash (nash at nrcbsa.bio.nrc.ca)
Institute for Biological Sciences, National Research Council of Canada,
Yet another Aussie-in-exile ;-)
*** Disclaimer: All opinions are mine, not NRC's! ***
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