disrupting e. coli cells

Basavaraju Shankarappa bsh at MED.PITT.EDU
Fri Mar 11 08:25:24 EST 1994


Try freezing and thawing couple of (several?) times with some liquid
nitrogen and a water bath.  You should get a much more efficient
disruption of e.coli.
Raj Shankarappa
bsh at med.pitt.edu
> 
> i've never had much success with sonication but since that is the only
> piece of equipment we have at present, do you have any suggestions for
> optimizing e. coli disruption with a sonicator? i've heard that some
> people use lysozyme treatment before sonicating, do you have any
> experience with this?? any other treatments??
> 
> bip
> -- 
> bipin k. dalmia               the other night i was lying on my bed, looking
> bipin at iastate.edu             up at the beautiful stars, and i said to myself, 
> n2.bkd at isumvs.iastate.edu     'where the F*CK is my ROOF !!'
> --
> 




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