PCR on phage

Martin Kennedy mkennedy at chmeds.ac.nz
Sun Mar 13 05:09:49 EST 1994

In article <1994Mar9.141913.3799 at wisipc.weizmann.ac.il>, Gustavo Glusman <bmgustav at dapsas1.weizmann.ac.il> writes:
> Is there anybody who could give me some advice on how to run PCR directly on
> the supernatant contains phage particle?
> Thanks!
I've had success just taking a small amount of raw lysate and adding it to the
PCR; from memory, I think I used 1ul and 0.1ul of the crude lysate obtained
from a phage plug (and eluted in a 1ml volume of phage buffer).  Less would
probably work fine too.  It wasn't necessary to boil or otherwise treat the



NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ
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