Cell surface Biotinylation

[Peter Smith]

Hi Folks, 
        Does anybody out there have a good method for labelling cell 
surface molecules with biotin? I've been using 125I labelling with pretty 
good results, but my finger tips are getting fried with all that 
radiation!  I'm sure that labelling the cell surface with biotin is 
possible....all I need is someone to tell me how!! Also any useful tips 
or pitfalls would be appreciated, I want to immunoprecipitate from the 
cells once they are labelled, will this be a problem?
        Thanks in advance from me and my finger tips!


Theresa

email: rtfm at cix.compulink.co.uk

Sunley Building
Kennedy Institute of Rheumatology
London
UK