Cell surface Biotinylation

Peter Smith rtfm at cix.compulink.co.uk
Sat Mar 12 16:39:04 EST 1994

Hi Folks, 
        Does anybody out there have a good method for labelling cell 
surface molecules with biotin? I've been using 125I labelling with pretty 
good results, but my finger tips are getting fried with all that 
radiation!  I'm sure that labelling the cell surface with biotin is 
possible....all I need is someone to tell me how!! Also any useful tips 
or pitfalls would be appreciated, I want to immunoprecipitate from the 
cells once they are labelled, will this be a problem?
        Thanks in advance from me and my finger tips!


email: rtfm at cix.compulink.co.uk

Sunley Building
Kennedy Institute of Rheumatology

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