Degenerate PCR

Seth Findley findley at u.washington.edu
Mon Mar 14 11:47:01 EST 1994


Does anybody out there have experience with doing PCR with highly 
degenerate oligo pairs?
I was thinking degeneracy on the order of 128 or 256 complexity.

(I have a number of "A or C or G or T" positions when I reverse 
translate a segment of a protein I am looking for homologs of)

Is this commonly problematic?
Does one have to add much more of the primer to make up for the complexity,
or do you just go a few more cycles?

Any tips greatly appreciated from people who have had success with this.

Thanks,

SDF



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