problem thermal cyclers (techne>

Grant McKegney mckegney at unixg.ubc.ca
Mon Mar 14 20:44:09 EST 1994


Our lab Techne III thermal cycler now fails to maintain (and sometimes
achieve) the programmed temperatures.  No longer can we cool a sample
below 14 degrees C.  If we wish to anneal at, say 57 degrees for 30 sec,
the actual temps would be:
59 degrees (start timing+0)
56 degrees t=10
56.2 degrees t=20
56.5 degrees t=30
So temp would never actually stabilize at 57.

Question: does anyone else have/had this problem with this model?  Is it
fixable, or do we just replace it?




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