plasmid integrated or not ?
Theo at mendel.Berkeley.EDU
Mon Mar 14 19:48:58 EST 1994
The following question was posted to me by a friend. I told
him to use inverse PCR (i.e. shearing the DNA into small fragments
-> self ligation -> inverse PCR -> subcloning and sequencing
-> designing new primers -> PCR across the junction betwwen
the vector and genomic sequence using unsheared genomic DNA
as templates ). Does anyone on the net have better
How can you test if the plasmid vector is integrated into
the genome of transfected cells ? (Only 5% of cells are
transfected and there's no selection marker on the vector).
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