Silver staining with ampholytes

Mon Mar 14 18:10:03 EST 1994

Hi, Lyndon-
I do silver staining of both SDS gels and IEF gels.  The only basic difference
between the two procedures is that with IEF, I first fix the proteins in
20% TCA.  Then the gel is washed in 10% ETOH-5% HAc (which is my first step
for an SDS gel).  Pharmacia sells a silver stain kit which I have used for
both of these procedures, but I now make up my own reagents and they work
just as well.  I suspect that your problem may be solved by first fixing
your proteins with TCA, then using your current silver stain procedure.

Hope this helps.


More information about the Methods mailing list