dsDNA site mutations

Tony Sanchez tsanchez at eye1.eye.ufl.edu
Tue Mar 15 14:36:23 EST 1994


I usually clone into a phagemid which allows ssDNA production (pTZ18 or 19U).  
But, I evaluated a Stratagene Doubletake kit once and it worked fine.  If I had a 
choice I would clone into a phagemid but if that was not a choice I would use the 
kit.
Tony Sanchez
University of Florida
Dept. Ophthalmology
1600 SW Archer Road
JHMHC #100284
ARB R4-146
Gainesville, FL 32610
Phone (904)392-2720
Fax (904)392-7839
tsanchez at eye1.eye.ufl.edu



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