plastic disposable columns

[Allison Hanly]

On 14 Mar 1994 15:33:48 GMT, 
Song Tan  <tan at aeolus.vmsmail.ethz.ch> wrote:


>Slight tangent to this discussion, but has anyone successfully used these
>BioRad biospin columns with plasmid DNA?  I use Sepharose CL-4B or S400 spun
>columns as the last step in my plasmid minipreps but when I tried using these
>resins in the BioRad biospin columns, I got no DNA back in the spun column
>"eluent".  Recovery from the home made spun columns (siliconized glass wool to
>plug a Gilson blue tip) was fine, so I attributed the loss to the biospin
>columns.  Would be nice to hear others' experiences.
>
What rotor type did you use?
Boehringer sell a similar column packed with sephadex for probe purification
their protocol uses swing out buckets rather than a fixed angle rotor.

Excerpt from product spec sheet

" In a fixed angle rotor, the sample is likely to slide down the sides of the tube instead of
flowing through the medium. This results in poor retention of nucleotides and decreased recovery of
sample "

Just a thought

Allison