Plasmid extraction

Julie Forney Menin forney at neb.com
Wed Mar 16 16:57:54 EST 1994


In article <1994Mar8.173858.16584 at rockyd.rockefeller.edu>,
ralston at rockyj.rockefeller.edu (Diana Ralston) wrote:

> >Is there anyone out there that can give me some advice on plasmid extraction
> >protocols of large plasmids i.e 30 kb and over.
>  I think it was something like Courrier and 
> Nester.  Also, I have used a protocol that originated from the
> Ausubel lab at Harvard.
> 
> Hope this helps,
> Diana Horvath
> ralston at rockvax.rockefeller.edu

Analytical Biochemistry 76:431-441 (1976)
Isolation of Covalently Closed Circular DNA of High Molecular Weight from
Bacteria
Thomas C. Currier and Eugene W. Nester



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