Plasmid extraction

Julie Forney Menin forney at
Wed Mar 16 16:57:54 EST 1994

In article <1994Mar8.173858.16584 at>,
ralston at (Diana Ralston) wrote:

> >Is there anyone out there that can give me some advice on plasmid extraction
> >protocols of large plasmids i.e 30 kb and over.
>  I think it was something like Courrier and 
> Nester.  Also, I have used a protocol that originated from the
> Ausubel lab at Harvard.
> Hope this helps,
> Diana Horvath
> ralston at

Analytical Biochemistry 76:431-441 (1976)
Isolation of Covalently Closed Circular DNA of High Molecular Weight from
Thomas C. Currier and Eugene W. Nester

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