{DNA isolation from plant cell

ez008413 at dale.ucdavis.edu ez008413 at dale.ucdavis.edu
Thu Mar 17 02:21:23 EST 1994


Could anybody please help me with DNA isolation from plant culture cells? 
I have tried the CTAB method as usually done for plant tissues eg. 
leaves, although I did not freeze the cells in liquid nitrogen prior to 
grinding.  I used about 7 g fresh weight of the culture cells (rice) but 
didn't see any sign of yield after addition of isopropanol.  I haven't 
proceeded since.  

Thanks for any assistance.


Gabby Romero
UC Davis



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