Loss of plasmid in liquid culture

Anne Savitt asavitt at sunysb.edu
Wed Mar 16 13:21:14 EST 1994


Bill Studier!s paper on the pET vectors (Meth. Enzym. 185:60-89) points
out that using ampicillin for selection has inherent problems because
beta lactamase is secreted into the medium and can destroy all of the
ampicillin, and there can be enough in an overnight culture to wipe out
all of the ampicillin in the larger culture at the time of inoculation. 
The paper discusses various ways around the problem of plasmid
instability.  There might be something in there to help with your problem.

Anne Savitt
Department of Microbiology
SUNY at Stony Brook
Stony Brook, NY   11794-5222
email:  asavitt at sunysb.edu



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