2D gel HELP !

Siggi Engelbrecht engel at ax3301.biologie.uni-osnabrueck.de
Thu Mar 17 05:02:00 EST 1994

Try to include 8M Urea in the first dimension and increase your protein  
concentration. You need A LOT to see bands! Try to get hold of the 
Pharmacia Phast System instruction manual, it includes a pretty good 
protocol for 2D electrophoresis and, although it's made for the 
Phast system, should be adjustable.

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