Freezing PCR products
Paul N Hengen
pnh at fcsparc6.ncifcrf.gov
Thu Mar 17 18:25:09 EST 1994
In article <2lpdbt$8v2 at mserv1.dl.ac.uk>
bss032 at vaxa.bangor.ac.uk writes:
>>A colleague of mine says that freezing a PCR product makes it disappear.
>>Does anyone have any information on this subject? The topic came up when a
>>PCR product was frozen prior to gel analysis. My colleague attributed the
>>absence of product to freezing. Naturally we are testing it. The
>>identical reaction did work a few days ago.
>
> Yes, but, dancing naked around the freezer makes it reappear :-).
I just did that experiment! Wouldn't you know it...the DNA was still there,
but all my labmates disappeared ;-) *POOF* like magic.
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* Paul N. Hengen, Ph.D. /--------------------------/*
* National Cancer Institute |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology | Phone: (301) 846-5581 |*
* Frederick Cancer Research and Development Center| FAX: (301) 846-5598 |*
* Frederick, Maryland 21702-1201 USA /--------------------------/*
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