Freezing PCR products

Paul N Hengen pnh at fcsparc6.ncifcrf.gov
Thu Mar 17 18:25:09 EST 1994


 In article <2lpdbt$8v2 at mserv1.dl.ac.uk>
 bss032 at vaxa.bangor.ac.uk writes:

>>A colleague of mine says that freezing a PCR product makes it disappear.
>>Does anyone have any information on this subject?  The topic came up when a
>>PCR product was frozen prior to gel analysis.  My colleague attributed the
>>absence of product to freezing.  Naturally we are testing it.  The
>>identical reaction did work a few days ago.
>
> Yes, but, dancing naked around the freezer makes it reappear :-).

I just did that experiment! Wouldn't you know it...the DNA was still there,
but all my labmates disappeared ;-) *POOF* like magic.

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* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
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