use of Pfu versus Taq: conditions?

rdkortschak at science.adelaide.edu.au rdkortschak at science.adelaide.edu.au
Tue Mar 15 17:19:18 EST 1994


Hi, does anyone know what the optimal conditions for Pfu DNA amplification ;-)
relative to using Taq (e.g. what is the extension rate of Pfu in nucleotides
per minute). One of our graduate students is attempting to construct specific
gene deletions using DNA amplification; she has got it to work using Taq but is
having problems with Pfu. Any help would be greatly appreciated.

thanks in advance. Daniel Kortschak
University of Adelaide, Genetics Department.




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