Isolating High MW DNA from Gel- Best Way??

Alejandro Abuin aabuin at bcm.tmc.edu
Thu Mar 17 17:12:23 EST 1994



     I have found the best way (in my hands) is to do a regular geneclean
(or prep-a gene, or ?.. you know, NaI + glassmilk + new wash...etc) BUT TO
DO ALL THE WASHINGS AND EVERY SINGLE STEP ON THE TIGHT GLASSMILK PELLET,
WITHOUT VORTEXING IT..

    I do this routinely and I have had no problems isolating clean (no
visible smear) 14Kb bands...

    Good luck


     



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