Sequencing PCR products
Shiao Y. Wang
sywang at whale.st.usm.edu
Sat Mar 19 00:28:54 EST 1994
I'm looking for people's experience with sequencing PCR products. Several
years ago, I tried sequencing a gel-purified restriction fragment
(approx 1.5 kb) WITHOUT success but WAS successful using the plasmid
clone as template. I reasoned that I was not able to keep the gel-pure
linear DNA denatured long enough or complete enough for the
primers to anneal.
Would I not run into the same problem with PCR products? Would cycle
sequencing get around this problem?
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