Poor capillary transfer?

[Tony Sanchez]

		Until we get our vacuum blotter, we are transfering our northerns
using 10xSSC capillary transfer overnite. But what we are finding is that
even after 16 hr transfer, only the 18S band is transfering, while the
higher stuff is staying very nicely in the gel. I have made sure that
there are enough paper towels and enough buffer in the reservoir. I was
thinking that maybe the weight I put on top of the paper towels is too
heavy, thus crushing the gel and preventing transfer of HMW bands. Any
other ideas?

thanxk
dlf
Doug Feinstein																	 |  Voice:   212 570-2900
Dept Neurobiology															|	Fax:      212 988-3672
Cornell University Medical College    |  E-mail: dlfeins at cumc.cornell.edu
We're not the best at what we do, but we're the only ones who do it...

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have you thought about trying  downwards blotting.   A collegue of mine uses it 
regulary for Northerns with great success.  A reference for this is Biotechniques 
16:58-59 (1994).
Tony Sanchez
University of Florida  Dept. Ophthalmology  1600 SW Archer Road
JHMHC #100284  Gainesville, FL 32610
Phone (904)392-2720      Fax (904) 392-7839           tsanchez at eye1.eye.ufl.edu