use of Pfu versus Taq: conditions?

Michael Benedik bchs1b at Elroy.UH.EDU
Fri Mar 18 17:11:16 EST 1994


In article <1994Mar15.171918.1 at science.adelaide.edu.au>, rdkortschak at science.adelaide.edu.au writes:
>Hi, does anyone know what the optimal conditions for Pfu DNA amplification ;-)
>relative to using Taq (e.g. what is the extension rate of Pfu in nucleotides
>per minute). One of our graduate students is attempting to construct specific
>gene deletions using DNA amplification; she has got it to work using Taq but is
>having problems with Pfu. Any help would be greatly appreciated.
>
>thanks in advance. Daniel Kortschak
>University of Adelaide, Genetics Department.
>
 In our hands raising the Mg++ concentration from 2mM to 3mM has made 
a world of difference in amplifying some things

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 Michael Benedik				INTERNET: Benedik at uh.edu
 Dept. of Biochemical & Biophysical Sciences	
 University of Houston				BITNET: Benedik at uhou
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