How low can you go (Nuc Acid pellets)?
James B. Hutchins
jbh at anat.UMSMED.EDU
Sat Mar 19 13:43:33 EST 1994
In article <2md9fd$qi7 at lastactionhero.rs.itd.umich.edu>
davidlee at whitman.ccs.itd.umich.edu (David Sang-shin Lee) writes:
>
>I was wondering....When precipitating DNA or RNA, how little nucleic acid
>can you have before the pellet (after centrifugation) becomes so small
>it's invisible?
>
I have never systematically studied this, but I do know that my wife and
I call these invisible entities "faith pellets" :-)
I hate faith pellets; I use tRNA or glycogen whenever possible, but there
are many, many situations where these coprecipitants cannot be used.
Jim
--
Jim Hutchins [] E-Mail: jbh at anat.umsmed.edu
Asst Prof of Anatomy [] Asst Prof of Neurology
Univ Mississippi Med Ctr [] Jackson, MS
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