Loss of plasmid in liquid culture

Duncan Clark Duncan at genesys.demon.co.uk
Sun Mar 20 12:48:35 EST 1994


Hi Folks,

If you have problems with ampicillin try using carbenicillin or a mixture
of amp plus methicillin (sorry I don't know the ratio - it is in the 
Stratagene pCRScript kit). These alternative B-lactams are far more 
resistant to B-lactamase than amp. This means that your O/N culture stands
a better chance of being 100% plasmid. Better still use kan. It isn't 
destroyed and therefore maintains continuous selection. Kan can also be 
used on high copy no. plasmids (unlike tet).

Regards

Duncan
 
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Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
GeneSys Ltd.                        | Compuserve:  100015.1406 at compuserve.com
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