Re. : promoter for Ampicillin in plasmids

[GWDGV1::KCHOWDH]

Hi Kaimin,
you can delete the ssp - sac fragment from pBS without any affect on the amp.
selection. you  can also  use other plasmids not containing the lacz region.
In such cases, you can select for the sucessfull insertion of lacZ by X-Gal
staining of the transformants. Cheers, Kamal.
Kamal Chowdhury
Max Planck Institute
Dept. Mol. Cell Biology
Am Fassberg
37077 Goettingen
Germany
Tel. (49) 551-201507
Fax. (49) 551-201504