Re. : promoter for Ampicillin in plasmids

GWDGV1::KCHOWDH kchowdh at gwdgv1.dnet.gwdg.de
Sun Mar 20 08:22:13 EST 1994


Hi Kaimin,
you can delete the ssp - sac fragment from pBS without any affect on the amp.
selection. you  can also  use other plasmids not containing the lacz region.
In such cases, you can select for the sucessfull insertion of lacZ by X-Gal
staining of the transformants. Cheers, Kamal.
Kamal Chowdhury
Max Planck Institute
Dept. Mol. Cell Biology
Am Fassberg
37077 Goettingen
Germany
Tel. (49) 551-201507
Fax. (49) 551-201504



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