Influenza HA tag
garrisonp at THORIN.UTHSCSA.EDU
Mon Mar 21 00:24:55 EST 1994
In Article <erman-170394210002 at 184.108.40.206>, erman at brandeis.edu.bitnet
(batu erman) wrote:
>I would like to tag the transcription factor that I work with, with an
>influenza HA tag. Does anyone know:
>1. where to get antibody for this epitope
>2. whether I should tag at the 5' or 3' end (the DNA binding domain is at
> end of the protein)
>3. is there a sequence that the antibody recognizes better than others.
You can get a polyclonal anti HA antibody from:
Babco Antibody Co.
4131 Lakeside Dr. Suite B
Richmond, CA 94806-1965
These people used to supply the monoclonal, but they didn't renew their license
from Scripps Clinic to keep doing so. Someone else may have taken their place-
you'll have to find out from Scripps. Richard Lerner and Ian Wilson were on
the original paper (Cell 37: 767 1984)- address:
Dept of Mol. Biol.
Research Inst of Scripps Clinic
La Jolla, CA 92037
I don't have a phone#.
I have used the monoclonal from Babco to detect a C-terminal 9 aa tag
(YPYDVPDYA). I could detect the epitope, but there was strong non-specific
protein binding that I never could succeed in blocking. Another person in our
dept. had the same experience. However, I know that other people have had no
problem with it, so it may have been a bad batch or it may not store well.
I have not used the polyclonal.The antibody can bind to the epitope on
either N or C terminus- both have been done. The original peptide epitope
is internal in the influenza hemaglutinin. For a good general account of
tagging, see Kolodziej, PA and Young, RA, Methods in Enzymol 194: 508 1991.
Hope this helps,
San Antonio, Tx 78284-7760
garrisonp at uthscsa.edu
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