Expression of fusion proteins

Alan H. Schulman schulman at
Mon Mar 21 15:43:44 EST 1994

I am interested in expressing a protein with an affinity tag which can later 
be cleaved off.  The cleavage should be specific, and not generate 
microheterogeneity from slight variations in position.  If you have experience 
with the various systems now available (such as Hisx6, DDDDK enterokinase 
cleavage (Invitrogen), strep-tag (Medical Braun), or Promega's Clip, I be 
interested in hearing.

Alan Schulman
University of Helsinki
schulman at
fax +358 0 434 6046

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