Salmon vs. Herring Testes
stahara at xlate.hsc.usc.edu
Mon Mar 21 12:51:33 EST 1994
In article <2m2dto$kia at nermal.cs.uoguelph.ca> rgiroux at uoguelph.ca (Randal W Giroux) writes:
>I have a fridge full of Herring Testes DNA (Sodium salt) from sigma. We
>are currently using the SAlmon testes version in our Southerns but wonder
>if we might use up the Herring Version. Is there a difference. A short
>investigation showed both have the same CAS number in the Sigma catalogue.
>Any insight would be appreciated. Does one function better than the other?
>If not why is Herring cheaper in the Sigma Catalogue?? Thanks in advance..
We had a similar question a few years back. In our hands we had
very poor luck with the herring DNA. It appeared that it had been
extensively degraded before we even got a chance to shear it. I see
no reason why herring DNA shouldn't be okay, providing it is of high MW.
We probably got a bad batch from Sigma. As a result of this we
alway buy the Salmon sperm DNA.
Hope this is useful.
|Stan Tahara Dept. of Microbiology |
|Phone: (213)-342-1722 USC School of Medicine |
|FAX: (213)-342-1721 stahara at xlate.hsc.usc.edu |
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