sequencing 60 bp?

Martin Leach leach at mbcrr.harvard.edu
Tue Mar 22 00:31:59 EST 1994


In article <1994Mar22.032655.1049590 at sue.cc.uregina.ca>,
ZACHARIAS at Meena.CC.URegina.CA (Tracey Zacharias) wrote:

> I have PCR'd out a fragment that is approximately 60 bp.  I am quite 
> sure that it is not just one fragment that I can sequence directly
> but that it is instead a multitude of fragments.  I will have to 
> subclone it into a vector of some sort and sequence several of the 
> subclones.  
> 
> There are several methods for sequencing now - I am interested in 
> kind of situation.  
>

I hope you are not going to subclone 60bp

I would suggest cloning it!

Clone em using a T-vector prep and sequence a couple of dozen! 8)

 
> Tracey Zacharias
> Department of Biology
> University of Regina
> Regina, SK  Canada
> Zacharit at max.cc.uregina.ca
> hearing what methods people are using and what is suggested for this	

-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
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