How low can you go (Nuc Acid pellets)?

Brian Foley brianf at
Mon Mar 21 17:37:51 EST 1994

David Sang-shin Lee (davidlee at wrote:
: I was wondering....When precipitating DNA or RNA, how little nucleic acid 
: can you have before the pellet (after centrifugation) becomes so small 
: it's invisible?

	It depends on how good your eyesight is, and how clean your DNA 
is.  Often times the pellet I see is mostly "other stuff" and not DNA.  I 
know that when I use real pure DNA for sequencing I cannot see the pellet 
when I have precipitated 5 micrograms of plasmid.  

: -------------------------------------------------------------------
: David S. Lee  (davidlee at                   All opinions
: HR6028, Kresge Hearing Research Institute             expressed
: University of Michigan                                 here are
: Ann Arbor MI  48109-0506                                mine!

*  Brian Foley               *     If we knew what we were doing   *
*  Molecular Genetics Dept.  *     it wouldn't be called research  *
*  University of Vermont     *                                     *

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