FUNGAL RNA EXTRACTION
obrien at CSUVAX1.MURDOCH.EDU.AU
Tue Mar 22 02:56:36 EST 1994
We have compared a number of methods for extraction of RNA from the
fungus Rhizoctonia solani. One method we found worked well is described
in Logemann et al 1987 Anal Biochem 163,16-20. Another method which also
worked was described by DeVries et al. 1988 Plant Mol. Biol. Manual Eds
Gelvin, Schilperoort, and Verma, Kluwer Academic Publishers Boston. Both
methods resulted in RNA yields of 150-250 ug/gmycelium. The critical
thing in isolating the RNA is to very quickly transfer the mycelium from
the growth medium into liquid nitrogen. Then quickly extract the RNA
observing the precautions normally observed for working with RNA.
Philip O'Brien Murdoch Univ Western Australia.
More information about the Methods