Deletions by site directed mutagenesis

ZURLINDEN at IMB.unibe.ch ZURLINDEN at IMB.unibe.ch
Tue Mar 22 04:25:31 EST 1994


Has anyone ever done some base deletions by site directed mutagenesis?
How many bases can be deleted at one time? How long should be the 
corresponding oligo?
In "Current Protocols", they write about 3-base deletions. Has somebody
tried this out sucessfully?
Thanks for your advices!

Andreas



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