transfecting COS cells

Val Snewin vsnewin at
Tue Mar 22 14:09:36 EST 1994

Does anyone have experience transfecting COS cells with the Gibco products,
LipofectAMINE, Lipofectin or LipofectACE ? I have been using DEAE-Dextran 
and electroporation but I only obtain about 10 % transfection (according to

B-gal staining). If anyone has a good protocol for any of the above, I 
would be very happy to hear from you.

Thanks in advance
Val Snewin


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