dsDNA site mutations

Sue Jeffrey sjeffrey.mskcc.org
Tue Mar 22 09:40:12 EST 1994


Todd-I've had a lot of luck with Clontech's mutagenesis kit, 
"Transformer". It is based on disrupting a site within the parental
clone to eliminate any non-mutated background, and it relies solely on 
T4 DNA polymerase, thus eliminating the disadvantages of PCR.  I
haven't tried Stratagene's kit, but it is more $$.  email me if you
want more info.
PS Hi Tony, how's life after BMS aka Hades?

Sue Jeffrey
sjeffrey at mskcc.org

What do I know, I'm only a tech!



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