Yes, we do deletions all the time with the USB T7-GEN Kit. We have done up to 50-base deletions in-house and our customers have done over 100-base deletions. If you design the oligo right (at least 15 matched bases on each side of the deletion) it works well. P. Flick -- Parke Flick, Ph.D. U.S. Biochemical Corp. Cleveland, OH 44128