G418

Hanson Lab Lab_Mac_Hanson at QMrelay.mail.cornell.edu
Wed Mar 23 08:29:18 EST 1994


In article <2movq6$n6k at mserv1.dl.ac.uk>, Graham Atherton
<GRGGTA at PICR.CR.MAN.AC.UK> wrote:

> G418 is used exclusively for selection of eukaryotic cell lines containing
> the G418/Kan - resistance gene. Kanamycin is only effective against
> procaryotic cell lines.


Also kan is used as a selective agent in plant transformations.  Is there
actually any data that kan is ineffective in eukaryotic selections?  I 
only remember the assertion that G418 was necessary for selection using the
nptII gene.   

Claudia



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