Inverse PCR

Matthias Zeiner mzei at sun0.urz.uni-heidelberg.de
Wed Mar 23 07:05:21 EST 1994


"Inverse PCR" is a method that should allow the amplification of the 
upstream and downstream regions flanking a DNA of known sequence. Briefly, 
the source DNA is circularized and PCR is carried out using primers in the 
opposite orientations to those normally employed for PCR. (Innis et al., 
PCR Protocols, A Guide to Methods and Applications, 1990).

My question is: Did anybody ever try this technique to complete cDNA's at 
                their 5'ends?
                Could this be an alternative to "RACE"?

Matthias Zeiner
Inst. Biol. Chem.
INF 501
D-69120 Heidelberg
mzei at sun0.urz.uni-heidelberg.de



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