HELP - Taq versus Pfu

NEAL MUSTO nmusto01 at GUMEDLIB.DML.GEORGETOWN.EDU
Wed Mar 23 17:58:45 EST 1994


We have used pfu in place of TAQ when we want fidelity. In our case we just
decreased the annealing temperature around 5-10 degrees. Be sure to use the
use the buffers supplied with the enzyme, not the TAQ buffer. TAQ buffer has
sodium salts at 50 mM, which is detrimental to pfu.
Good luck
Neal Musto



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