HELP - Taq versus Pfu

jsecombe at jsecombe at
Sat Mar 19 10:08:19 EST 1994

HELP - I have been using PCR to generate specific deletions in a gene and have
 got the reaction to work well using Taq polymerase.  I can't get the reaction
to work using Pfu enzyme.
Has anyone used Pfu - and if so, what reaction conditions need to be changed
compared with Taq to get optimal DNA amplification.

Any help would be valued.

Julie Secombe
University of Adelaide, Dept of Genetics.

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