GeneAmp RNA PCR kit questions
J. Logan Black
Black.John at mayo.edu
Fri Mar 25 12:24:27 EST 1994
I am using the above referenced kit to look for a low copy number mRNA expressed in small cell
lung carcinoma and I am having problems getting the kit to work. I start by DNasing the sample
with an RNase free DNase I (Boehringer Mannheim) 6U/mcg total RNA at 37C for 1 hour then heat inactivate the
DNase to 90C x 9min. I then follow the kit's recommendations through completion of the PCR.
Net result is nothing on the 1.5% agarose gel.
Is anybody else using this kit with success? Any 'tricks'?
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