** DEPC and reagents **

Ding Ming ming at ahabs.wisc.edu
Fri Mar 25 16:39:55 EST 1994


In article <9403252029.AA18997 at pines2.ljcrf.edu>, helgew at LJCRF.EDU (Helge
Weissig) wrote:

> 
>         does anyone know the exact rxn mechanism of DEPC (any approximation
> thereof would be appriciated as well :))?

I am assuming you are talking about diethylpyrocarbonate(DEPC), right?:)

If so, the reaction is a carboxyethlation reaction on N position(if it is a
amino group). The nucleophiles can be others, like His, Cys, tyrosine etc.



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Ding Ming
University of Wisconsin-Madison
Telephone: (608)265-3544     Fax: (608)262-7420
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