looking for a mice heart cell line
mfreis at lsumc.edu
Fri Mar 25 18:38:46 EST 1994
>> I am looking for a mice heart cell line, more specifically a cardiac
>>muscle cell line. I looked up the catalog from American Type Culture
>>, and I couldn't find one. The closest one is a rat heart cell line
>>, but it behaves like a skeletal muscle, so I am not sure whether I shall use
>> I will greatly appreciate your help and any suggestion on this matter.
>>mman at molbio.cbs.umn.edu
Look up the work from William Claycomb, Joe Del Carpio and Lauren Field.
They use atrial myocytes from transgenic mice that have an atrial-specific
promotoer driving the SV40 T Ag. They have developed culture conditions
where the cells beat in culture, and express atrial-specific markers. They
have carried the lines for four years.
More information about the Methods