coady at ERE.UMontreal.CA
Thu Mar 24 23:30:27 EST 1994
In article <2mtb3r$e70 at gazette.bcm.tmc.edu> wzhao at mbcr.bcm.tmc.edu writes:
>Is there anybody who has been using XL1Blue MRF' a lot? I had some
>problems with the strain. Can anybody tell me the optimal concentration
>of tetracycline for growing this strain, and a optimal protocol for
>making competent cells.
>Thanks in advance!
Yes, I've been using them a fair bit; I don't recall the
tet concentration offhand (and it's not very close to this lab
at the moment) but it's what was recommended in "Current Protocols",
so it can't be too odd. I use the method of Inoue et al (Gene,
sometime in 1990) to prepare the bacteria and I get 2.5 x 10E8
per ug. Feel free to email me with any specific questions about
them. Oh, and I am in no way connected with Stratagene save being
an occasional customer.
Michael J. Coady
COADY at ERE.UMONTREAL.CA
The opinions expressed above are solely those of the author and do not,
in any way, shape or form, represent the Universite de Montreal.
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