blunt-ending PCR product

mcgraw at BSCR.UGA.EDU mcgraw at BSCR.UGA.EDU
Fri Mar 25 17:07:59 EST 1994


Hi,

does anyone out there care to recommend or reference a sure-fire method for
making a PCR product ready for ligation with a linker via blunt-end ligation?
In other work, we've used primers with restriction sites and we've done T-A
cloning also...this is something different...we really need to ligate linkers
to PCR products via blunt-end ligation.  Any suggestions?

Thanks,
	Al McGraw



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