disrupting e. coli cells

Bill Jack jack at neb.com
Fri Mar 25 14:59:52 EST 1994


In article <2lsssf$kim at columba.udac.uu.se>, dunten at sol.bmc.uu.se (Pete
Dunten) wrote:

> 
> 3 volumes buffer to 1 gram (wet) cells works pretty well.  
> You can see the cell suspension change color when the cells
> break.  Or you can put a few microliters on a slide, cover
> with a coverslip, and take a look under a microscope.  Intact
> cells and broken cell envelopes can be distinguished, so you
> can decide when to stop sonicating.
Alternatively, take a small aliquot and spin out the debris. Measure
protein in the supernatant using a Bradford assay. When the protein
concentration doesn't increase with increasing sonication, the cells are
completely broken

Bill Jack
New England Biolabs
jack at neb.com  



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